Facile Attachment of TAT Peptide on Gold Monolayer Protected Clusters: Synthesis and Characterization
نویسندگان
چکیده
High affinity thiolate-based polymeric capping ligands are known to impart stability onto nanosized gold nanoparticles. Due to the stable gold-sulfur bond, the ligand forms a protective layer around the gold core and subsequently controls the physicochemical properties of the resultant nanogold mononuclear protected clusters (AuMPCs). The choice of ligands to use as surfactants for AuMPCs largely depends on the desired degree of hydrophilicity and biocompatibility of the MPCs, normally dictated by the intended application. Subsequent surface modification of AuMPCs allows further conjugation of additional biomolecules yielding bilayer or multilayered clusters suitable for bioanalytical applications ranging from targeted drug delivery to diagnostics. In this study, we discuss our recent laboratory findings on a simple route for the introduction of Trans-Activator of Transcription (TAT) peptide onto the surface of biotin-derivatised gold MPCs via the biotin-strepavidin interaction. By changing the surface loading of biotin, controlled amounts of TAT could be attached. This bioconjugate system is very attractive as a carrier in intercellular delivery of various delivery cargoes such as antibodies, proteins and oligonucleotides.
منابع مشابه
Synthesis, Separation, and Characterization of Polymers from Gold Monolayer Protected Clusters
متن کامل
Porphyrin metal complex monolayer-protected gold nanorods: a parallel facile synthesis and self-assembly.
Porphyrin metal (Zn, Cu, and Mg) complex monolayer-protected gold nanorods (GNRs) were, for the first time, synthesized. Their synthesis was easy to access by mixing porphyrin encapsulated GNRs with corresponding excess soluble metal salts in solution, followed by the facile purification through centrifugation and sonication due to the gravity of the GNRs and their solubility in organic solvent...
متن کاملA facile transformation of amino acids to functionalized coumarins.
The synthesis of novel chiral coumarins functionalized with proteinogenic amino acid side chains via N-protected γ-amino-β-keto esters and their incorporation into the cell permeable HIV-1 TAT peptide through the modified solid phase peptide synthesis are described.
متن کاملSub-nanometer Au monolayer-protected clusters exhibiting molecule-like electronic behavior: quantitative high-angle annular dark-field scanning transmission electron microscopy and electrochemical characterization of clusters with precise atomic stoichiometry.
The synthesis and characterization of the clusters Au13[PPh3]4[S(CH2)11CH3]2Cl2 (1) and Au13[PPh3]4[S(CH2)11CH3]4 (2) are described. These mixed-ligand, sub-nanometer clusters, prepared via exchange of dodecanethiol onto phosphine-halide gold clusters, show enhanced stability relative to the parent. The characterization of these clusters features the precise determination of the number of gold ...
متن کاملSynthesis, Surface Assembly, Characterization and Electrochemistry of Gold Nanoparticles
Aalto University, P.O. Box 11000, FI-00076 Aalto www.aalto.fi Author Outi Toikkanen Name of the doctoral dissertation Synthesis, Surface Assembly, Characterization and Electrochemistry of Gold Nanoparticles Publisher School of Chemical Technology Unit Department of Chemistry Series Aalto University publication series DOCTORAL DISSERTATIONS 19/2011 Field of research Physical Chemistry Manuscript...
متن کامل